I'm not understanding the question. You can program the chip on your desktop PC and then re-install it, or oyu can use a Laptop in your car with it connected to the EEC.
What I meant was does the EEC need to be connected to a 12V power source (in the car with the 60-pin connector) to be able to tune it with the TwEECer? Or, can I completely remove the EEC and tune it with my desktop computer?