Major difference is firing order. non-HO is 1-5-4... HO is 1-3-7...
Some non-HO motors have roller cams esp. the late model Crown Vics. Most have flat top pistons which limits your cam lift to basically stock
If you have an Non-HO motor in an HO Mustang, you need to swap the camshaft to an HO cam or it won't run right